2026-04-17T04:13:21Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/4155572026-03-31T11:55:47Zcom_2072_1033col_2072_452950

00925njm 22002777a 4500 dc Castro Carrasco, Rebeca Ignacia author Gabriel Buguña, Gemma author Gabriel Buguña, David author Gamisans Noguera, Javier author Guimerà Villalba, Xavier author 2024-06 The sulfate-reduction process plays a crucial role in the biological valorization of SOx gases. However, a complete understanding of the sulfidogenic process in bioreactors is limited by the lack of technologies for characterizing the sulfate-reducing activity of immobilized biomass. In this work, we propose a flow-cell bioreactor (FCB) for characterizing sulfate-reducing biomass using H2S microsensors to monitor H2S production in real-time within a biofilm. To replace natural immobilization through extracellular polymeric substance production, sulfidogenic sludge was artificially immobilized using polymers. Physical and sulfate-reducing activity studies were performed to select a polymer-biomass matrix that maintained sulfate-reducing activity of biomass while providing strong microbial retention and mechanical strength. Several operational conditions of the sulfidogenic reactor allowed to obtain a H2S profiles under different inlet sulfate loads and, additionally, 3D mapping was assessed in order to perform a hydraulic characterization. Besides, the effects of artificial immobilization on biodiversity were investigated through the characterization of microbial communities. This study demonstrated the appropriateness of immobilized-biomass for characterization of sulfidogenic biomass in FCB using H2S electrochemical microsensors, and beneficial microbiological communities shifts as well as enrichment of sulfate-reducing bacteria have been confirmed. Grant PID2021-126253OB-C22 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. Peer Reviewed Postprint (published version) Àrees temàtiques de la UPC::Enginyeria química::Química del medi ambient Sulfate-reducing bacteria Biomass immobilization PVA Agar Alginate Electrochemical microsensor Development of a flow-cell bioreactor for immobilized sulfidogenic sludge characterization using electrochemical H2S microsensors