2026-04-13T02:48:37Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/3946012026-02-07T07:08:00Zcom_2072_1033col_2072_452950

00925njm 22002777a 4500 dc Gutierrez Capitan, Manuel author Sanchís Villariz, Ana author Carvalho, Estela author Baldi Coll, Antonio author Vilaplana Holgado, Lluïsa author Cardoso, Vanessa author Calleja Navalpotro, Álvaro author Wei, Mingxing author de la Rica, Roberto author Hoyo Pérez, Javier author Bassegoda Puigdomenech, Arnau author Tzanov, Tzanko author Marco Colas, María Pilar author Lanceros Méndez, Senentxu author Fernandez Sanchez, Cesar author 2023-07-19 Health initiatives worldwide demand affordable point- of-care devices to aid in the reduction of morbidity and mortality rates of high-incidence infectious and noncommunicable diseases. However, the production of robust and reliable easy-to-use diagnostic platforms showing the ability to quantitatively measure several biomarkers in physiological fluids and that could in turn be decentralized to reach any relevant environment remains a challenge. Here, we show the particular combination of paper-microfluidic technology, electro- chemical transduction, and magnetic nanoparticle-based immunoassay approaches to produce a unique, compact, and easily deployable multiplex device to simultaneously measure interleukin-8, tumor necrosis factor-a, and myeloperoxidase biomarkers in sputum, developed with the aim of facilitating the timely detection of acute exacerbations of chronic obstructive pulmonary disease. The device incorporates an on-chip electrochemical cell array and a multichannel paper component, engineered to be easily aligned into a polymeric cartridge and exchanged if necessary. Calibration curves at clinically relevant biomarker concentration ranges are produced in buffer and artificial sputum. The analysis of sputum samples of healthy individuals and acutely exacerbated patients produces statistically significant biomarker concentration differences between the two studied groups. The device can be mass-produced at a low cost, being an easily adaptable platform for measuring other disease-related target biomarkers. This research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) grants PCIN-2016-052 and PCIN-2016-134 and by Portuguese Fundação para a Ciência e a Technologia grant ENMed/0049/2016, through the ERA-NET EuroNanoMed II initiative (LungCheck project). This research was also funded by the European Commision-NextGenerationEU (Regulation EU 2020/2094), through CSIC’s Global Health Platform (PTI). This work used the Spanish ICTS Network MICRONANOFABS and was partly supported by the Spanish Ministry of Science and Innovation. Peer Reviewed Postprint (published version) Àrees temàtiques de la UPC::Enginyeria química Lungs -- Diseases, Obstructive Biosensors Bioelectronics Point-of-care rapid tests Chronic obstructive pulmonary disease Electrochemical biosensing Paper microfluidics Magnetic nanoparticle-based immunoassay Multiplexed detection Pulmons -- Malalties obstructives Biosensors Bioelectrònica Engineering a point-of-care paper-microfluidic electrochemical device applied to the multiplexed quantitative detection of biomarkers in sputum