2026-04-17T18:24:03Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/3879122026-02-11T05:19:30Zcom_2072_1033col_2072_452950

Multimodal silver-chitosan-acylase nanoparticles inhibit bacterial growth and biofilm formation by gram-negative pseudomonas aeruginosa bacterium Ferreres Cabanes, Guillem Ivanova, Kristina Dimitrova Torrent Burgués, Juan Tzanov, Tzanko Universitat Politècnica de Catalunya. Departament d'Enginyeria Química Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial Àrees temàtiques de la UPC::Enginyeria biomèdica Microbial biotechnology Biofilms Silver-chitosan nanoparticles Acylase Antimicrobial activity Quorum quenching Biofilm inhibition Biotecnologia microbiana Biofilms Pseudomonas aeruginosa bacteria originate severe infections in hospitalized patients and those with chronic debilitating diseases leading to increased morbidity and mortality, longer hospitalization and huge financial burden to the healthcare system. The clinical relevance of P. aeruginosa infections is increased by the capability of this bacterium to grow in biofilms and develop multidrug resistant mechanisms that preclude conventional antibiotic treatments. Herein, we engineered novel multimodal nanocomposites that integrate in the same entity antimicrobial silver nanoparticles (NPs), the intrinsically antimicrobial, but biocompatible biopolymer chitosan, and the anti-infective quorum quenching enzyme acylase I. Acylase present in the NPs specifically degraded the signal molecules governing bacterial cell-to-cell communication and inhibited by ~55 % P. aeruginosa biofilm formation, while the silver/chitosan template altered the integrity of bacterial membrane, leading to complete eradication of planktonic bacteria. The innovative combination of multiple bacteria targeting modalities resulted in 100-fold synergistic enhancement of the antimicrobial efficacy of the nanocomposite at lower and non-hazardous towards human skin cells concentrations, compared to the silver/chitosan NPs alone. Peer Reviewed Postprint (published version) 2023-05 Article Ferreres, G. [et al.]. Multimodal silver-chitosan-acylase nanoparticles inhibit bacterial growth and biofilm formation by gram-negative pseudomonas aeruginosa bacterium. "Journal of colloid and interface science", Maig 2023, vol. 645, p. 576-586. 0021-9797 https://hdl.handle.net/2117/387912 10.1016/j.jcis.2023.04.184 eng https://www.sciencedirect.com/science/article/pii/S0021979723007841 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104111RB-I00/ES/RECUBRIMIENTOS DE HIDROGEL QUE CONTIENEN NANOPARTICULAS ANTIMICROBIANAS PARA COMBATIR INFECCIONES ASOCIADAS AL USO DE CATETERES POR BACTERIAS RESISTENTES A LOS ANTIBIOTICOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2021-121926/ES/NANOENABLED STRATEGIES TO REDUCE THE PRESENCE OF CONTAMINANTS OF EMERGING CONCERN IN AQUATIC ENVIRONMENT/ http://creativecommons.org/licenses/by-nc-nd/4.0/ Open Access Attribution-NonCommercial-NoDerivatives 4.0 International 11 p. application/pdf