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00925njm 22002777a 4500 dc Gualda Manzano, Emilio José author Moreno, Nuno author 2015-08 Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional optical microscopy or scanning electron microscopy systems it offers the possibility of obtaining multi-views of the sample by rotating it. Here we show that the use of light sheet microscopy for the analysis of invertebrates provides a fair compromise compared to scanning electron microscopy (SEM) in terms of resolution but avoiding some its drawbacks such as sample preparation or limited three-dimensional perspectives Fundação para a Ciência e Tecnologia, grant SFRH/BD/80717/2011 Postprint (published version) Àrees temàtiques de la UPC::Ciències de la visió::Òptica física::Llum Fluorescence. Fluorescence microscopy Fluorescència 3D volume rendering of invertebrates using light-sheet fluorescence microscopy