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The neck region of the C-type lectin DC-SIGN regulates its surface spatiotemporal organization and virus-binding capacity on antigen-presenting cells* Manzo, Carlo Torreño Piña, Juan Andrés Joosten, Ben Reinieren Beeren, Inge Gualda Manzano, Emilio José Loza Álvarez, Pablo Figdor, Carl G. Garcia-Parajo, Maria F. Cambi, Alessandra Universitat Politècnica de Catalunya. Departament d'Enginyeria Agroalimentària i Biotecnologia Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia Dendritic Cells. Viral binding Cèl·lules dendrítiques fol·liculars The C-type lectin DC-SIGN expressed on dendritic cells(DCs) facilitates capture and internalization of a plethora of dif-ferent pathogens. Although it is known that DC-SIGN organizesin nanoclusters at the surface of DCs, the molecular mecha-nisms responsible for this well defined nanopatterning and rolein viral binding remain enigmatic. By combining biochemicaland advanced biophysical techniques, including optical super-resolution and single particle tracking, we demonstrate thatDC-SIGN intrinsic nanoclustering strictly depends on itsmolecular structure. DC-SIGN nanoclusters exhibited free,Brownian diffusion on the cell membrane Postprint (published version) 2012-11 Article Manzo, C. [et al.]. The neck region of the C-type lectin DC-SIGN regulates its surface spatiotemporal organization and virus-binding capacity on antigen-presenting cells*. "Journal of biological chemistry", Novembre 2012, vol. 287, núm. 46, p. 38946-38955. 0021-9258 https://hdl.handle.net/2117/344727 10.1074/jbc.m112.380121 eng https://www.jbc.org/article/S0021-9258(20)62356-0/fulltext http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Open Access Attribution-NonCommercial-NoDerivs 3.0 Spain 10 p. application/pdf