2026-04-17T07:58:17Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/209082026-02-07T09:29:23Zcom_2072_1033col_2072_452950

Co-assembled and microfabricated bioactive membranes Mendes, Ana C. Smith, Katherine H. Tejeda Montes, Esther Engel López, Elisabeth Reis, Rui L. Azevedo, Elena S. Mata, Álvaro Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica Institut de Bioenginyeria de Catalunya Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits Àrees temàtiques de la UPC::Enginyeria dels materials Biomedical materials biomaterials The fabrication of hierarchical and bioactive self-supporting membranes, which integrate physical and biomolecular elements, using a single-step process that combines molecular self-assembly with soft lithography is reported. A positively charged multidomain peptide (with or without the cell-adhesive sequence arginine-glycine-aspartic acid-serine (RGDS)) self- assembles with hyaluronic acid (HA), an anionic biopolymer. Optimization of the assembling conditions enables the realization of membranes with well-controlled and easily tunable features at multiple size scales including peptide sequence, building-block co-assembly, membrane thickness, bioac- tive epitope availability, and topographical pattern morphology. Membrane structure, morphology, and bioactivity are investigated according to tempera- ture, assembly time, and variations in the experimental setup. Furthermore, to evaluate the physical and biomolecular signaling of the self-assembled microfabricated membranes, rat mesenchymal stem cells are cultured on membranes exhibiting various densities of RGDS and different topographical patterns. Cell adhesion, spreading, and morphology are signifi cantly affected by the surface topographical patterns and the different concentrations of RGDS. The versatility of the combined bottom-up and top-down fabrication processes described may permit the development of hierarchical macrostruc- tures with precise biomolecular and physical properties and the opportunity to fi ne tune them with spatiotemporal control. Peer Reviewed Postprint (published version) 2012-09-03 Article Mendes, A. [et al.]. Co-assembled and microfabricated bioactive membranes. "Advanced functional materials", 03 Setembre 2012, vol. 28, p. 430-438. 1616-301X https://hdl.handle.net/2117/20908 10.1002/adfm.201201065 eng http://onlinelibrary.wiley.com/doi/10.1002/adfm.201201065/abstract http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Restricted access - publisher's policy Attribution-NonCommercial-NoDerivs 3.0 Spain 9 p. application/pdf