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Modular polylactic acid microparticle-based scaffolds prepared via microfluidic emulsion/solvent displacement process: Fabrication, characterization, and in vitro mesenchymal stem cells interaction study Salerno, Aurelio Levato, R. Mateos Timoneda, Miguel Ángel Engel López, Elisabeth Netti, Paolo Antonio Planell Estany, Josep Anton Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica Institut de Bioenginyeria de Catalunya Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits Àrees temàtiques de la UPC::Enginyeria química Bone regeneration. green solvent microfluidic microstructure stem cells scaffold Ossos -- Regeneració The present study reports a novel approach for the design and fabrication of polylactic acid (PLA) microparticle- based scaffolds with microstructural properties suitable for bone and cartilage regeneration. Macroporous PLA scaffolds with controlled shape were fabricated by means of a semicon- tinuous process involving (1) microfluidic emulsification of a PLA/ethyl lactate solution (5% w/v) in a span 80/paraffin oil so- lution (3% v/v) followed by (2) particles coagulation/assembly in an acetone/water solution for the development of a continu- ous matrix. Porous scaffolds prepared from particles with monomodal or bimodal size distribution, overall porosity ranges from 93 to 96%, interparticles porosity from 41 to 54%, and static compression moduli from 0.3 to 1.4 MPa were man- ufactured by means of flow rate modulation of of the continu- ous phase during emulsion. The biological response of the scaffolds was assessed in vitro by using bone marrow-derived rat mesenchymal stem cells (MSCs). The results demonstrated the ability of the scaffolds to support the extensive and uni- form three-dimensional adhesion, colonization, and prolifera- tion of MSCs within the entire construct Peer Reviewed Postprint (published version) 2012-08-31 Article Salerno, A. [et al.]. Modular polylactic acid microparticle-based scaffolds prepared via microfluidic emulsion/solvent displacement process: Fabrication, characterization, and in vitro mesenchymal stem cells interaction study. "Journal of biomedical materials research. Part A", 31 Agost 2012, vol. 101, núm. 3, p. 720-732. 1549-3296 https://hdl.handle.net/2117/19029 10.1002/jbm.a.34374 eng http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34374/full http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Restricted access - publisher's policy Attribution-NonCommercial-NoDerivs 3.0 Spain 13 p. application/pdf