2026-04-05T12:57:19Zhttps://recercat.cat/oai/requestoai:recercat.cat:2117/190292026-02-04T08:10:33Zcom_2072_1033col_2072_452950
RECERCAT
author
Salerno, Aurelio
author
Levato, R.
author
Mateos Timoneda, Miguel Ángel
author
Engel López, Elisabeth
author
Netti, Paolo Antonio
author
Planell Estany, Josep Anton
2012-08-31
The present study reports a novel approach for the
design and fabrication of polylactic acid (PLA) microparticle-
based scaffolds with microstructural properties suitable for
bone and cartilage regeneration. Macroporous PLA scaffolds
with controlled shape were fabricated by means of a semicon-
tinuous process involving (1) microfluidic emulsification of a
PLA/ethyl lactate solution (5% w/v) in a span 80/paraffin oil so-
lution (3% v/v) followed by (2) particles coagulation/assembly
in an acetone/water solution for the development of a continu-
ous matrix. Porous scaffolds prepared from particles with
monomodal or bimodal size distribution, overall porosity
ranges from 93 to 96%, interparticles porosity from 41 to 54%,
and static compression moduli from 0.3 to 1.4 MPa were man-
ufactured by means of flow rate modulation of of the continu-
ous phase during emulsion. The biological response of the
scaffolds was assessed
in vitro
by using bone marrow-derived
rat mesenchymal stem cells (MSCs). The results demonstrated
the ability of the scaffolds to support the extensive and uni-
form three-dimensional adhesion, colonization, and prolifera-
tion of MSCs within the entire constructPeer ReviewedPostprint (published version)
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Restricted access - publisher's policy Attribution-NonCommercial-NoDerivs 3.0 Spain
Àrees temàtiques de la UPC::Enginyeria química
Bone regeneration.
green solvent
microfluidic
microstructure
stem cells
scaffold
Ossos -- Regeneració
Modular polylactic acid microparticle-based scaffolds prepared via microfluidic emulsion/solvent displacement process: Fabrication, characterization, and in vitro mesenchymal stem cells interaction study
Article