2026-04-13T04:08:44Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/1843252024-05-29T16:27:44Zcom_2072_1033col_2072_452950

00925njm 22002777a 4500 dc Tornin Cavielles, Juan author Villasante, Aranzazu author Sole Marti, Xavi author Ginebra Molins, Maria Pau author Canal Barnils, Cristina author 2020 These data are associated to the paper with the same title of the authors, and are structured according to the figures in the paper: http://hdl.handle.net/2117/335727 The use of Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer’s Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future 1 http://hdl.handle.net/2117/184325 Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials Cold atmospheric plasma, Plasma activated liquids; Osteosarcoma, Cancer stem-like cells, 3D tumor model Osteosarcoma tissue-engineered model challenges oxidative stress therapy revealing promoted cancer stem cell properties [Dataset]