2026-04-14T03:14:50Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/1215142026-01-21T05:34:44Zcom_2072_1033col_2072_452950

urn:hdl:2117/121514 Bottom-up layer-by-layer assembling of antibacterial freestanding nanobiocomposite films Francesko, Antonio Ivanova, Kristina Dimitrova Hoyo Pérez, Javier Pérez-Rafael, Silvia Petkova, Petya Fernandes Macedo, Margarida Maria Heinze, Thomas Mendoza Gómez, Ernesto Tzanov, Tzanko Àrees temàtiques de la UPC::Enginyeria química Chitosan Biopolymers Nanoparticles--therapeutic use chitosan aminocellulose hyaluronic acid biopolyme r-capped silver 1 nanoparticles layer-by-layer freestanding antimicrobial films Quitosan Biopolímers Nanopartícules -- Ús terapèutic In this study, freestanding nanobiocomposite films were obtained by the sequential deposition of biopolymer-capped silver nanoparticles (AgNPs) and hyaluronic acid (HA). At first, dispersions ofAgNPs decorated with chitosan (CS) or aminocellulose (AC) were synthesized by applying high intensity ultrasound. These polycationic nanoentities were layer-by-layer assembled with the HA polyanion to generate stable 3D supramolecular constructs, where the biopolymer-capped AgNPs play the dual role of active agent and structural element. SEM images of the assemblies revealed gradual increase of thickness with the number of deposited bilayers. The composites of =50 bilayers were safe to human cells and demonstrated 100% antibacterial activity against Staphylococcus aureus and Escherichia coli. Moreover, the films containing CSAgNPs brought about the total prevention of biofilm formation reducing the cells surface adherence by up to 6 logs. Such nanobiocomposites could serve as an effective barrier to control bacterial growth on injured skin, burns, and chronic wounds. Peer Reviewed Postprint (author's final draft) 2018-07-27 Article https://pubs.acs.org/doi/10.1021/acs.biomac.8b00626 http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Open Access Attribution-NonCommercial-NoDerivs 3.0 Spain