2026-04-17T22:51:27Zhttps://recercat.cat/oai/request

oai:recercat.cat:2117/1132742026-02-02T10:11:31Zcom_2072_1033col_2072_452950

00925njm 22002777a 4500 dc Llucía Valldeperas, Aida author Bragós Bardia, Ramon author Soler Botija, Carolina author Roura Ferrer, Santiago author Gálvez Montón, Carolina author Prat Vidal, Cristina author Perea Gil, Isaac author Bayés Genis, Antoni author 2018-01-11 Mechanical conditioning is incompletely characterized for stimulating therapeutic cells within the physiological range. We sought to unravel the mechanism of action underlying mechanical conditioning of adipose tissue-derived progenitor cells (ATDPCs), both in vitro and in silico. Cardiac ATDPCs, grown on 3 different patterned surfaces, were mechanically stretched for 7 days at 1 Hz. A custom-designed, magnet-based, mechanical stimulator device was developed to apply ~10% mechanical stretching to monolayer cell cultures. Gene and protein analyses were performed for each cell type and condition. Cell supernatants were also collected to analyze secreted proteins and construct an artificial neural network. Gene and protein modulations were different for each surface pattern. After mechanostimulation, cardiac ATDPCs increased the expression of structural genes and there was a rising trend on cardiac transcription factors. Finally, secretome analyses revealed upregulation of proteins associated with both myocardial infarction and cardiac regeneration, such as regulators of the immune response, angiogenesis or cell adhesion. To conclude, mechanical conditioning of cardiac ATDPCs enhanced the expression of early and late cardiac genes in vitro. Additionally, in silico analyses of secreted proteins showed that mechanical stimulation of cardiac ATDPCs was highly associated with myocardial infarction and repair. Peer Reviewed Postprint (published version) Àrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica::Electrònica en cardiologia Electronics in cardiology Cardiology Stem cells Electrònica en cardiologia Unravelling the effects of mechanical physiological conditioning on cardiac adipose tissue-derived progenitor cells in vitro and in silico