2026-04-17T14:03:22Zhttps://recercat.cat/oai/requestoai:recercat.cat:2117/1041822025-07-17T12:17:27Zcom_2072_1033col_2072_452950
High-Resolution Morphological Approach to Analyse Elastic Laminae Injuries of the Ascending Aorta in a Murine Model of Marfan Syndrome
López-Guimet, Júlia
Andilla, Jordi
Loza-Álvarez, Pablo
Egea, Gustavo
Àrees temàtiques de la UPC::Física
Aorta
Marfan Syndrome
Fotònica
In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic
aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic
laminae. However, conventional histological techniques using transverse sections provide limited
information about the precise location, progression and 3D extension of the microstructural changes
that occur in each lamina. We implemented a method using multiphoton excitation fluorescence
microscopy and computational image processing, which provides high-resolution en-face images
of segmented individual laminae from unstained whole aortic samples. We showed that internal
elastic laminae and successive 2nd laminae are injured to a different extent in murine Marfan aortae;
in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were
concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the
waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is
a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative threedimensional
information about vascular tissue. The application of this method to murine Marfan aortae
clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness
of the tunica media and in the different anatomical regions of the ascending aorta.
Peer Reviewed
Postprint (published version)
2017-05-04
Article
https://www.nature.com/articles/s41598-017-01620-8
info:eu-repo/grantAgreement/EC/H2020/654148/EU/The Integrated Initiative of European Laser Research Infrastructures/LASERLAB-EUROPE
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Open Access
Attribution-NonCommercial-NoDerivs 3.0 Spain
Nature