2026-04-13T02:55:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4889922026-03-05T23:32:12Zcom_2072_98col_2072_378192

Biodiesel synthesis in a solvent-free system by recombinant Rhizopus oryzae Lipase : study of the catalytic reaction progress Canet Morral, Albert Benaiges, M. Dolors Valero Barranco, Francisco Matèries del catàleg Immobilization Biodiesel Monoacylglycerols Recombinant Rhizopus oryzae lipase Response surface model The recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL) was used to synthesize biodiesel and monoacylglycerols simultaneously. The reaction was carried out in a solvent-free system with the enzyme immobilized on octadecyl-Sepabeads. Using response surface methodology, the methyl ester yield was optimized by means of the study of the effect of water, substrate molar ratio (methanol:olive oil) and methanol stepwise addition. It was concluded that in order to prevent enzyme inactivation by methanol, alcohol should be added slowly; otherwise a large amount of water would be present. Taking the best conditions, a 50.3 % yield was achieved in 3 h, which corresponds to 75.4 % of the acyl groups at the 1,3-position undergoing transesterification. It was also concluded that methyl esters result from the esterification of the free fatty acid hydrolyzed by the enzyme and also from a direct transesterification of oil. In addition, the fatty acid selectivity of rROL was found not to favor one fatty acid in olive oil over another. 2014 Article Ministerio de Ciencia e Innovación CTQ2010-15131 Generalitat de Catalunya 2009/SGR-281 Generalitat de Catalunya 2010/CONE3-00063 Journal of the American Oil Chemists' Society ; Vol. 91, Num. 9 (2014), p. 1499-1506 open access Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets. https://rightsstatements.org/vocab/InC/1.0/