2026-04-13T06:44:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4871692025-10-27T13:52:38Zcom_2072_98col_2072_378192

Acute stress triggers sex-dependent rapid alterations in the human small intestine microbiota composition Rodiño-Janeiro, Bruno K. Khannous-Lleiffe, Olfat Pigrau Pastor, Marc Willis, Jesse R. Salvo-Romero, Eloísa Nieto Ruiz, Adoración Expósito, Elba Fortea, Marina Pardo-Camacho, Cristina Albert-Bayo, Mercé González Castro, Ana Maria Guagnozzi, Danila Martínez, Cristina Lobo Álvarez, Beatriz Vicario Perez, Maria Santos, Javier Gabaldón, Toni Alonso Cotoner, Carmen Universitat Autònoma de Barcelona Stress Functional dyspepsia Disorders of gut-brain interaction Irritable bowel syndrome Small intestine microbiota Digestive disorders of gut-brain interaction (DGBI) are very common, predominant in females, and usually associated with intestinal barrier dysfunction, dysbiosis, and stress. We previously found that females have increased susceptibility to intestinal barrier dysfunction in response to acute stress. However, whether this is associated with changes in the small bowel microbiota remains unknown. We have evaluated changes in the small intestinal microbiota in response to acute stress to better understand stress-induced intestinal barrier dysfunction. Jejunal biopsies were obtained at baseline and 90 min after cold pain or sham stress. Autonomic (blood pressure and heart rate), hormonal (plasma cortisol and adrenocorticotropic hormone) and psychological (Subjective Stress Rating Scale) responses to cold pain and sham stress were monitored. Microbial DNA from the biopsies was analyzed using a 16S metabarcoding approach before and after cold pain stress and sham stress. Differences in diversity and relative abundance of microbial taxa were examined. Cold pain stress was associated with a significant decrease in alpha diversity (P = 0.015), which was more pronounced in females, along with significant sex differences in the abundance of specific taxa and the overall microbiota composition. Microbiota alterations significantly correlated with changes in psychological responses, hormones, and gene expression in the intestinal mucosal. Cold pain stress was also associated with activation of autonomic, hormonal and psychological response, with no differences between sexes. Acute stress elicits rapid alterations in bacterial composition in the jejunum of healthy subjects and these changes are more pronounced in females. Our results may contribute to the understanding of female predominance in DGBI. 2025 Article Ministerio de Economía y Competitividad CD15/00010 Ministerio de Ciencia e Innovación CM10/00155 Ministerio de Economía y Competitividad FI12/00254 Instituto de Salud Carlos III PI19/01643 Ministerio de Economía y Competitividad PI16/00583 Ministerio de Economía y Competitividad CPII16/00031 Ministerio de Economía y Competitividad PI15/00301 Ministerio de Sanidad y Consumo CB06/04/0021 Frontiers in microbiology ; Vol. 15 (january 2025) open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/