2026-04-17T16:16:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4853832025-10-27T13:36:30Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Pesqué, David author Andrades, Evelyn author Berenguer-Molins, Pau author Perera-Bel, Júlia author Clarós, Miquel author Bódalo-Torruella, Marta author Gonzàlez-Farré, Mònica author Gallardo, Fernando author Pujol Vallverdú, Ramón M. author Giménez-Arnau, Ana M author 2025 Altres ajuts: acords transformatius de la UAB Altres ajuts: LEO Foundation (LF-OC-23-001209) Background: Immune mechanisms underlying elicitation in allergic contact dermatitis (ACD) have yet to be fully elucidated. Previous studies have shown a double-faceted nature of ACD with both common biomarkers among different allergens and allergen73 specific imprinting, albeit with discordance in terms of relevant pathways involved. Several factors, including co-existing atopic dermatitis, may influence immune reactions. We aim to characterize molecular signatures and their immune mechanisms of different relevant allergens (nickel, 2-hydroxyethylmethacrylate [2-HEMA], methylisothiazolinone [MIT], formaldehyde) in strong and extreme positive (2/3+) patch test reactions of patients without atopic dermatitis. Methods: A transcriptomic analysis of 40 skin biopsies of ACD reactions (11 nickel, 10 MIT, 10 2-HEMA, 9 formaldehyde) and 19 controls (petrolatum-occluded skin) was performed using RNA sequencing. Differentially-expressed genes (DEG) were assessed and enriched functional pathways were obtained with an over-representation analysis for allergens. Results: ACD molecular profiling revealed a strong, common imprinting of DEG among allergens versus controls (n=814), with further partially-shared DEG among allergens (n=664) and allergen-specific DEG (n=430). The most relevant shared pathways were associated with immune adaptive and innate responses. All allergens exhibited mixed effector immune responses, mainly type 1 and 3 immunity, and, to a lesser extent, type 2 immunity. Furthermore, partially-shared and unique DEG were associated with further inflammatory pathways, particularly for nickel and 2-HEMA. Conclusions: This study confirms shared ACD imprinting among different allergens and shared pathways' predominant role in ACD elicitation in patients without atopic dermatitis, alongside allergen-specific immune processes and mixed effector responses (type 1, 3 and 2). Contact dermatitis Allergic Transcriptomic Immune Patch test Transcriptomic analysis of allergic patch test reactions in non-atopic patients: a comparative study across multiple allergens