2026-04-18T07:51:46Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4851412025-08-31T18:15:57Zcom_2072_98col_2072_378192

The long-term in vitro co-exposure of polyethylene terephthalate (PET) nanoplastics and cigarette smoke condensate exacerbates the induction of carcinogenic traits Morataya-Reyes, Michelle Villacorta, Aliro Gutiérrez-García, Javier Egea, Raquel Martín-Pérez, Joan Barguilla, Irene Marcos Dauder, Ricardo Hernández Bonilla, Alba Nanoplastics Cigarette smoke condensate Long-term co-exposure Beas-2B cells Carcinogenic traits Altres ajuts: acords transformatius de la UAB This study examines the long-term impact of polyethylene terephthalate nanoplastics (PET-NPLs) and cigarette smoke condensate (CSC) on human lung BEAS-2B cells, focusing on key biological hallmarks of carcinogenesis. True-to-life PET-NPLs were generated from plastic water bottles and characterized to simulate environmental exposure conditions; and a comprehensive battery of assays was employed to assess genotoxicity, cellular transformation, and invasiveness. It was observed that, compared to passage control and individual exposures, co-exposure to PET-NPLs and CSC exacerbates oxidative stress, genotoxicity, and tumorigenic transformation, as evidenced by increased DNA damage, colony formation in soft agar, and enhanced cell migration and invasion. Transcriptomic analysis revealed a shift in cellular stress regulation including the upregulation of stress-response genes, including SLC7A11, NQO1, and HSPA1A, which are linked to oxidative stress adaptation and tumor survival. At the same time, key tumor-suppressor genes, such as LOX, and FN1, were significantly downregulated, promoting cellular transformation and invasiveness. These results provide compelling evidence that the combination of PET-NPLs and CSC enhances carcinogenic traits through oxidative stress, genomic instability, and disruption of tumor-suppressive pathways. This study underscores the importance of evaluating the synergistic effects of combined environmental exposures and their implications for human health. 2025 Article https://ddd.uab.cat/record/311524 urn:10.1016/j.jhazmat.2025.138359 urn:oai:ddd.uab.cat:311524 urn:scopus_id:105003110010 urn:articleid:18733336v493p138359 urn:oai:egreta.uab.cat:publications/ffdeb043-099a-4c65-b86c-dcf6c3b8b8cc http://hdl.handle.net/2072/485141 eng Agència de Gestió d'Ajuts Universitaris i de Recerca 2023/BP-00212 European Commission 965196 Agencia Estatal de Investigación PID2020-116789RB-C43 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731 Journal of hazardous materials ; Vol. 493 (August 2025), art. 138359 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ application/pdf