2026-04-13T15:06:26Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4840262025-08-31T18:08:46Zcom_2072_98col_2072_378192

urn:hdl:2072/484026 Peripheral and intestinal T lymphocyte subsets in dogs with chronic inflammatory enteropathy Agulla, Beatriz Villaescusa, Alejandra Sainz Rodríguez, Ángel Díaz-Regañón, David Rodríguez Franco, Fernando Calleja-Bueno, Lydia Olmeda, Patricia García-Sancho, Mercedes Flow cytometry IBD Intraepithelial lymphocytes Lamina propria lymphocytes Peripheral blood lymphocytes T lymphocytes Altres ajuts: Proyecto Santander de la Universidad Complutense de Madrid (PR 26/-16-20319; PR 75/18-21623; PR 87/19-22606). Dysregulated T lymphocyte response is thought to play a key role in chronic intestinal inflammation (CIE). To evaluate the presence of changes in peripheral and intestinal T lymphocyte subsets and to describe potential immune and inflammatory biomarkers in dogs with CIE. Sixteen healthy dogs and 26 dogs were diagnosed with CIE. Prospective case-control study evaluating peripheral and intestinal T lymphocytes using flow cytometry and inflammatory markers obtained from complete blood cell counts. Dogs with CIE had higher peripheral activated T helper (Th) lymphocytes (87/μL [18-273] CIE, 44/μL [16-162] healthy control (HC, P = .013) and regulatory T cells (Treg; 108/μL [2-257] CIE, 34/μL [1-114] HC, P = .004). In the intestinal epithelium, CIE dogs presented lower percentages of Th (4.55% [1.75-18.67] CIE, 8.77% [3.79-25.03] HC, P = .002), activated Th cells (0.16% [0.02-0.83] CIE, 0.33% [0.05-0.57] HC, P = .03) and CD4/CD8 ratio (0.08 [0.02-0.39] CIE, 0.21 [0.07-0.85] HC, P = .003). Conversely, higher percentage of activated T cytotoxic cells (20.24% [3.12-77.12] CIE, 12.32% [1.21-39.22] HC, P = .04) and interferon-gamma (IFN-γ) producing T lymphocytes (7.36% [0.63-55.83] CIE, 1.44% [0.00-10.56] HC, P = .01) within the epithelium was observed. In the lamina propria the percentage of Treg lymphocytes was higher (6.02% [1.00-21.48] CIE, 3.52% [0.18-10.52] HC, P = .02). Systemic and intestinal immune alterations occur in dogs with CIE suggesting that blood IFN-γ producing T lymphocytes and the systemic immune-inflamation index (SII) could potentially serve as biomarkers for the disease. 2024 Article Journal of Veterinary Internal Medicine ; Vol. 38 (May/June 2024), p. 1437-1448 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. https://creativecommons.org/licenses/by-nc-nd/4.0/