2026-04-14T02:06:04Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4766912025-04-03T10:58:01Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Samouillan, Valerie author García Rodríguez, Jesús Eduardo author Benitez Amaro, Aleyda author La Chica Lhoëst, Maria Teresa author Dandurand, Jany author Actis Dato, Virginia author Guerra Ramos, José María author Escolà-Gil, Joan Carles author Chiabrando, Gustavo author Enrich, Carlos author Llorente-Cortes, Vicenta author Universitat Autònoma de Barcelona author 2023 The accumulation of lipids in cardiomyocytes contributes to cardiac dysfunction. The specific blockage of cardiomyocyte cholesteryl ester (CE) loading by antibodies (Abs) against the P3 sequence (Gly 1127 -Cys 1140) of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 Abs on high-fat diet (HFD)-induced cardiac extracellular matrix (ECM) biophysical alterations was analyzed. Both IrP (without Abs) and P3-immunized rabbits (with Abs) were randomized into groups fed either HFD or a standard chow diet. Cardiac lipids, proteins, and carbohydrates were characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance mode. The hydric organization and physical structure were determined by differential scanning calorimetry. HFD increased the levels of esterified lipids, collagen, and α-helical structures and upregulated fibrosis, bound water, and ECM plasticization in the heart. The inhibitory effect of anti-P3 Abs on cardiac CE accumulation was sufficient to reduce the collagen-filled extracellular space, the level of fibrosis, and the amount of bound water but did not counteract ECM plasticization in the heart of hypercholesterolemic rabbits. Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia