2026-04-14T02:04:32Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4742872025-07-29T22:42:39Zcom_2072_98col_2072_378192

urn:hdl:2072/474287 Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease Garrido-Trigo, Alba Corraliza, Ana Maria Veny, Marisol Dotti, Isabella Melón-Ardanaz, Elisa Rill, Aina Crowell, Helena L. Corbí, Ángel Gudiño, Victoria Esteller, Miriam Álvarez-Teubel, Iris Aguilar, Daniel Masamunt, M.Carmen Killingbeck, E. Kim, Youngmi Leon, Michael Visvanathan, Sudha Marchese, Domenica Caratù, Ginevra Martin Cardona, Albert Esteve, Maria Panés, Julián Ricart, Elena Mereu, Elisabetta Heyn, Holger Salas, Azucena Crohn Disease Humans Inflammatory Bowel Diseases Macrophages Neutrophils RNA Ulcerative colitis and Crohn's disease are chronic inflammatory intestinal diseases with perplexing heterogeneity in disease manifestation and response to treatment. While the molecular basis for this heterogeneity remains uncharacterized, single-cell technologies allow us to explore the transcriptional states within tissues at an unprecedented resolution which could further understanding of these complex diseases. Here, we apply single-cell RNA-sequencing to human inflamed intestine and show that the largest differences among patients are present within the myeloid compartment including macrophages and neutrophils. Using spatial transcriptomics in human tissue at single-cell resolution (CosMx Spatial Molecular Imaging) we spatially localize each of the macrophage and neutrophil subsets identified by single-cell RNA-sequencing and unravel further macrophage diversity based on their tissue localization. Finally, single-cell RNA-sequencing combined with single-cell spatial analysis reveals a strong communication network involving macrophages and inflammatory fibroblasts. Our data sheds light on the cellular complexity of these diseases and points towards the myeloid and stromal compartments as important cellular subsets for understanding patient-to-patient heterogeneity. 2023 Article European Commission 848028 European Commission 831434 Agencia Estatal de Investigación PID2020-115439GB-I00 Agencia Estatal de Investigación RTI2018-096946-B-I00 Nature communications ; Vol. 14 Núm. 1 (december 2023), p. 4506 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/