2026-04-17T20:31:53Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4706202024-11-26T02:12:00Zcom_2072_98col_2072_378192

A Comparative Study Using Fluorescent Confocal Microscopy and Flow Cytometry to Evaluate Chondrocyte Viability in Human Osteochondral Allografts López-Chicón, Patricia Riba-Tietz, Tatiana Fariñas Barbera, Oscar Gelber Ghertner, Pablo Eduardo Casaroli-Marano, Ricardo Pedro Vilarrodona, Anna Universitat Autònoma de Barcelona Osteoarthritis Osteochondral grafts Chondrocytes cell viability Graft preservation Confocal fluorescence microscopy Flow cytometry The preservation conditions of fresh osteochondral allografts for clinical applications are critical due their objective: to transplant mature hyaline cartilage containing viable chondrocytes, maintaining their metabolic activity and also preserving the structural and functional characteristics of the extracellular matrix. The aim of the present study was to compare fluorescence confocal microscopy and flow cytometry techniques to evaluate the viability of the chondrocytes present in the osteochondral tissue, in order to determine their effectiveness and thus ensure reproducibility and robustness of the analysis. To this end, osteochondral allografts from human cadaveric donors were preserved at 4 °C for 3 weeks in a preservation medium supplemented with antibiotic and antifungal agents. Cell viability of chondrocytes was determined by monitoring the cartilage for 3 weeks of preservation by confocal fluorescence microscopy and flow cytometry, obtaining cell viabilities of 83.7 ± 2.6% and 55.8 ± 7.8% for week three, respectively. The confocal fluorescence microscopy approach is more advantageous and accurate, as it correlates better with actual cell viability values for monitoring osteochondral graft preservation, detecting only the cells that died a natural death associated with the preservation method. 2022 Article https://ddd.uab.cat/record/283217 urn:10.3390/bioengineering9100515 urn:oai:ddd.uab.cat:283217 urn:scopus_id:85140376271 urn:articleid:23065354v9n10p515 urn:pmid:36290483 urn:pmc-uid:9598719 urn:pmcid:PMC9598719 urn:oai:pubmedcentral.nih.gov:9598719 urn:oai:egreta.uab.cat:publications/36ea9f28-0c0a-4c13-8b8e-d41865675b94 eng Bioengineering ; Vol. 9 Núm. 10 (october 2022), p. 515 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ application/pdf