2026-04-18T06:08:17Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4665152024-11-04T05:20:18Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc D'Acunzo, Pasquale author Pérez-González, Rocío author Kim, Yohan author Hargash, Tal author Miller, Chelsea author Alldred, Melissa J. author Erdjument-Bromage, Hediye author Penikalapati, Sai Charan author Pawlik, Monika author Saito, Mitsuo author Saito, Mariko author Ginsberg, Stephen D. author Neubert, Thomas A. author Goulbourne, Chris N. author Levy, Efrat author 2021 Altres ajuts: National Institute on Aging (AG017617, AG056732, and AG057517); National Institute on Drug Abuse (DA044489); NIH Instrumentation Grant (S10RR027990). Mitochondrial dysfunction is an established hallmark of aging and neurodegenerative disorders such as Down syndrome (DS) and Alzheimer's disease (AD). Using a high-resolution density gradient separation of extracellular vesicles (EVs) isolated from murine and human DS and diploid control brains, we identify and characterize a previously unknown population of double-membraned EVs containing multiple mitochondrial proteins distinct from previously described EV subtypes, including microvesicles and exosomes. We term these newly identified mitochondria-derived EVs "mitovesicles." We demonstrate that brain-derived mitovesicles contain a specific subset of mitochondrial constituents and that their levels and cargo are altered during pathophysiological processes where mitochondrial dysfunction occurs, including in DS. The development of a method for the selective isolation of mitovesicles paves the way for the characterization in vivo of biological processes connecting EV biology and mitochondria dynamics and for innovative therapeutic and diagnostic strategies. http://hdl.handle.net/2072/466515 Alzheimer's disease Biological process Density gradient separation Diagnostic strategy Mitochondrial dysfunction Mitochondrial protein Neurodegenerative disorders Selective isolation Alzheimer Disease Animals Down Syndrome Exosomes Extracellular Vesicles Humans Mice Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in down syndrome