2026-04-18T07:26:35Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4656542024-11-04T05:04:42Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc G. Rico, Laura author Salvia, Roser author Ward, Michael D. author Bradford, Jolene A. author Petriz, Jordi author 2021 This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis. For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018). http://hdl.handle.net/2072/465654 Flow cytometry/Mass cytometry Cancer Health sciences Clinical protocol Immunology Stem Cells Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation