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oai:recercat.cat:2072/4656542024-11-04T05:04:42Zcom_2072_98col_2072_378192

2024-11-04T05:04:42Z urn:hdl:2072/465654 Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation G. Rico, Laura Salvia, Roser Ward, Michael D. Bradford, Jolene A. Petriz, Jordi Flow cytometry/Mass cytometry Cancer Health sciences Clinical protocol Immunology Stem Cells This protocol provides instructions to improve flow cytometry analysis of marrow/peripheral blood cells by avoiding erythrolytic solutions, density gradients, and washing steps. We describe two basic approaches for identifying cell surface antigens with minimal sample perturbation, which have been successfully used to identify healthy and pathologically rare cells. The greatest advantage of these approaches is that they minimize the unwanted effect caused by sample preparation, allowing for improved study of live cells at the point of analysis. For complete details on the use and execution of this protocol, please refer to Petriz et al. (2018). 2024-11-04T05:04:42Z 2024-11-04T05:04:42Z 2021 Altres http://hdl.handle.net/2072/465654 STAR Protocols ; Vol. 2 Núm. 4 (17 2021), art. 100883 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. https://creativecommons.org/licenses/by-nc-nd/4.0/