2026-04-17T14:05:00Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4654112026-03-28T08:14:28Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Jiménez de la Morena, Jorge author Conesa Mingo, Pablo author Fonseca-Reyna, Yunior César author de Isidro-Gómez, Federico P. author Herreros, David author Fernández-Giménez, Estrella author Střelák, David author Moebel, Emmanuel author Buchholz, Tim Oliver author Jug, Florian author Martínez-Sánchez, Antonio author Harastani, Mohamad author Jonic, Slavica author Conesa, José Javier author Cuervo, Ana author Losana, Patricia author Sánchez-Caballero, Ignacio author Iceta Tena, Mikel author del Cano, Laura author Gragera, Marcos author Melero, Roberto author Sharov, Grigory author Castaño-Díez, Daniel author Koster, Abraham J. author Piccirillo, Jonathan G. author Vilas, Jose Luis author Oton, Joaquin author Marabini, Roberto author Sorzano, Carlos Oscar S. author Carazo Garcia, José María author 2022 Image processing in cryogenic electron tomography (cryoET) is currently at a similar state as Single Particle Analysis (SPA) in cryogenic electron microscopy (cryoEM) was a few years ago. Its data processing workflows are far from being well defined and the user experience is still not smooth. Moreover, file formats of different software packages and their associated metadata are not standardized, mainly since different packages are developed by different groups, focusing on different steps of the data processing pipeline. The Scipion framework, originally developed for SPA (), has a generic python workflow engine that gives it the versatility to be extended to other fields, as demonstrated for model building (). In this article, we provide an extension of Scipion based on a set of tomography plugins (referred to as ScipionTomo hereafter), with a similar purpose: to allow users to be focused on the data processing and analysis instead of having to deal with multiple software installation issues and the inconvenience of switching from one to another, converting metadata files, managing possible incompatibilities, scripting (writing a simple program in a language that the computer must convert to machine language each time the program is run), etcetera. Additionally, having all the software available in an integrated platform allows comparing the results of different algorithms trying to solve the same problem. In this way, the commonalities and differences between estimated parameters shed light on which results can be more trusted than others. ScipionTomo is developed by a collaborative multidisciplinary team composed of Scipion team engineers, structural biologists, and in some cases, the developers whose software packages have been integrated. It is open to anyone in the field willing to contribute to this project. The result is a framework extension that combines the acquired knowledge of Scipion developers in close collaboration with third-party developers, and the on-demand design of functionalities requested by beta testers applying this solution to actual biological problems. ScipionTomo : Towards cryo-electron tomography software integration, reproducibility, and validation