2026-04-14T02:35:54Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4618032025-06-12T09:13:47Zcom_2072_98col_2072_378192

Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein Martínez-Orellana, Pamela González, Noemí Baldassarre, Antonella Álvarez-Fernández, Alejandra Ordeix, Laura (Ordeix i Esteve) Paradies, Paola Soto, Manuel Solano Gallego, Laia Leishmaniosis Serology Dog Cell-mediated immunity Diagnosis The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11. 2022 Article https://ddd.uab.cat/record/257221 urn:10.3390/vetsci9030116 urn:oai:ddd.uab.cat:257221 urn:articleid:23067381v9n3p116 urn:pmid:35324844 urn:pmc-uid:8954708 urn:pmcid:PMC8954708 urn:oai:pubmedcentral.nih.gov:8954708 urn:oai:egreta.uab.cat:publications/fe4374f8-8333-416d-bc84-b15d9756a36d urn:scopus_id:85126706486 eng Ministerio de Economía y Competitividad AGL2015-68477 Veterinary sciences ; Vol. 9 Núm. 3 (2022), p. 116 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ application/pdf