2026-04-17T05:16:48Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4592292026-03-28T07:34:04Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Martinez de Lejarza Samper, Ignacio Miguel author Benitez Amaro, Aleyda author Viladés Medel, David author Dandurand, Jany author Casas, Josefina author Jorge, Esther author Gonzalo Calvo, David de author Gallardo, Alberto author Lerma Puertas, Enrique author Guerra Ramos, José María author Carreras Costa, Francesc author Leta, Rubén author Llorente-Cortés, Vicenta author Universitat Autònoma de Barcelona author 2020 Altres ajuts: Fundació La MARATÓ de TV3 (201516_10) Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography-high-resolution mass spectrometry, LC-HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans. Biophysical markers Cardiac remodeling post-MI Lipidomics Fourier transform infrared spectroscopy Heart failure Biophysical and Lipidomic Biomarkers of Cardiac Remodeling Post-Myocardial Infarction in Humans