2026-04-13T02:32:22Zhttps://recercat.cat/oai/request
oai:recercat.cat:2072/4588332026-03-28T07:31:31Zcom_2072_98col_2072_378192
RECERCAT author de Luna Salva, Noemí author Turon-Sans, Janina author Cortés-Vicente, Elena author Carrasco-Rozas, Ana author Illán-Gala, Ignacio author Dols Icardo, Oriol author Clarimón, Jordi author Lleó, Alberto author Gallardo, Eduard author Illa, Isabel author Rojas-Garcia, Ricardo author Universitat Autònoma de Barcelona 2020 Altres ajuts:Fundació La Marató de TV3 (201437.10 to R.R.). Rio Hortega grant (CM17/00074) from "Acción Estratégica en Salud 2013-2016"Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease for which the pathophysiological mechanisms of motor neuron loss are not precisely clarified. Environmental and epigenetic mechanisms such as microRNAs (miRNAs) could have a role in disease progression. We studied the expression pattern of miRNAs in ALS serum from 60 patients and 29 healthy controls. We also analyzed how deregulated miRNAs found in serum affected cellular pathways such as apoptosis, autophagy and mitochondrial physiology in SH-SY5Y cells. We found that miR-335-5p was downregulated in ALS serum. SH-SY5Y cells were transfected with a specific inhibitor of miR-335-5p and showed abnormal mitochondrial morphology, with an increment of reactive species of oxygen and superoxide dismutase activity. Pro-apoptotic caspases-3 and 7 also showed an increased activity in transfected cells. The downregulation of miR-335-5p, which has an effect on mitophagy, autophagy and apoptosis in SH-SY5Y neuronal cells could have a role in the motor neuron loss observed in ALS. open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ Neurological disorders Neurodegenerative diseases Neuromuscular disease Downregulation of miR-335-5P in Amyotrophic Lateral Sclerosis Can Contribute to Neuronal Mitochondrial Dysfunction and Apoptosis Article