2026-04-17T06:48:21Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4587252024-11-12T00:41:40Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Van Eeckhout, Albert author Garcia-Caurel, Enrique author Garnatje, Teresa author Escalera Merino, Juan Carlos author Durfort, Mercè author Vidal, Josep author Gil Pérez, José Jorge author Campos Coloma, Juan author Lizana Tutusaus, Ángel author 2021 Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques. Imaging and sensing Plant physiology Stomata Polarization microscopy Polarimetric imaging microscopy for advanced inspection of vegetal tissues