2026-04-18T07:54:12Zhttps://recercat.cat/oai/request
oai:recercat.cat:2072/4585922024-11-01T00:59:12Zcom_2072_98col_2072_378192
RECERCAT author Vidal Barba, Enric author Burgaya, Judit author Michelet, Lorraine author Arrieta-Villegas, Claudia author Cantero, Guillermo author de Cruz, Krystel author Tambosco, Jennifer author di Bari, Michele author Romolo, Nonno author Boschiroli, Maria Laura author Pérez de Val, Bernat 2024-11-01T00:59:12Z 2024-11-01T00:59:12Z 2022 http://hdl.handle.net/2072/458592 Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis / M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (10 5 and 10 6 CFU/0.1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles. open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ Voles Wild rodents Tuberculosis Mycobacterium microti Experimental infection Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus) Article