2026-04-20T00:12:01Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4581182025-05-28T03:37:53Zcom_2072_98col_2072_378192

Human-induced neural and mesenchymal stem cell therapy combined with a curcumin nanoconjugate as a spinal cord injury treatment Bonilla, Pablo Hernández Martín, Joaquim Giraldo, Esther González-Pérez, Miguel A. Alastrue-Agudo, Ana Elkhenany, Hoda Vicent, María J. Navarro, X. (Xavier) Edel, Michael J. Moreno-Manzano, Victoria Spinal cord injury Stem cells Curcumin Neuroprotection Polymer-drug conjugate Altres ajuts: Fundació Marató TV3 2017/refs.20172230, 20172231 i 20172110 We currently lack effective treatments for the devastating loss of neural function associated with spinal cord injury (SCI). In this study, we evaluated a combination therapy comprising human neural stem cells derived from induced pluripotent stem cells (iPSC-NSC), human mesen-chymal stem cells (MSC), and a pH-responsive polyacetal-curcumin nanoconjugate (PA-C) that al-lows the sustained release of curcumin. In vitro analysis demonstrated that PA-C treatment pro-tected iPSC-NSC from oxidative damage in vitro, while MSC co-culture prevented lipopolysaccha-ride-induced activation of nuclear factor-κB (NF-κB) in iPSC-NSC. Then, we evaluated the combination of PA-C delivery into the intrathecal space in a rat model of contusive SCI with stem cell transplantation. While we failed to observe significant improvements in locomotor function (BBB scale) in treated animals, histological analysis revealed that PA-C-treated or PA-C and iPSC-NSC + MSC-treated animals displayed significantly smaller scars, while PA-C and iPSC-NSC + MSC treatment induced the preservation of β-III Tubulin-positive axons. iPSC-NSC + MSC transplantation fostered the preservation of motoneurons and myelinated tracts, while PA-C treatment polarized microglia into an anti-inflammatory phenotype. Overall, the combination of stem cell transplantation and PA-C treatment confers higher neuroprotective effects compared to individual treatments. 2021 Article Agencia Estatal de Investigación RTI2018-095872-B-C21 International journal of molecular sciences ; Vol. 22, Issue 11 (June 2021), art. 5966 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/