2026-04-14T09:40:58Zhttps://recercat.cat/oai/requestoai:recercat.cat:2072/4581182025-05-28T03:37:53Zcom_2072_98col_2072_378192
RECERCAT
author
Bonilla, Pablo
author
Hernández Martín, Joaquim
author
Giraldo, Esther
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González-Pérez, Miguel A.
author
Alastrue-Agudo, Ana
author
Elkhenany, Hoda
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Vicent, María J.
author
Navarro, X. (Xavier)
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Edel, Michael J.
author
Moreno-Manzano, Victoria
2021
Altres ajuts: Fundació Marató TV3 2017/refs.20172230, 20172231 i 20172110We currently lack effective treatments for the devastating loss of neural function associated with spinal cord injury (SCI). In this study, we evaluated a combination therapy comprising human neural stem cells derived from induced pluripotent stem cells (iPSC-NSC), human mesen-chymal stem cells (MSC), and a pH-responsive polyacetal-curcumin nanoconjugate (PA-C) that al-lows the sustained release of curcumin. In vitro analysis demonstrated that PA-C treatment pro-tected iPSC-NSC from oxidative damage in vitro, while MSC co-culture prevented lipopolysaccha-ride-induced activation of nuclear factor-κB (NF-κB) in iPSC-NSC. Then, we evaluated the combination of PA-C delivery into the intrathecal space in a rat model of contusive SCI with stem cell transplantation. While we failed to observe significant improvements in locomotor function (BBB scale) in treated animals, histological analysis revealed that PA-C-treated or PA-C and iPSC-NSC + MSC-treated animals displayed significantly smaller scars, while PA-C and iPSC-NSC + MSC treatment induced the preservation of β-III Tubulin-positive axons. iPSC-NSC + MSC transplantation fostered the preservation of motoneurons and myelinated tracts, while PA-C treatment polarized microglia into an anti-inflammatory phenotype. Overall, the combination of stem cell transplantation and PA-C treatment confers higher neuroprotective effects compared to individual treatments.
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Spinal cord injury
Stem cells
Curcumin
Neuroprotection
Polymer-drug conjugate
Human-induced neural and mesenchymal stem cell therapy combined with a curcumin nanoconjugate as a spinal cord injury treatment
Article