2026-04-17T16:56:52Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4578952026-03-13T07:50:41Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc López-Laguna, Hèctor author Sanchez, Julieta M. author Carratalá, José Vicente author Rojas Peña, Mauricio author Sánchez-García, Laura author Parladé Molist, Eloi author Sánchez Chardi, Alejandro author Voltà-Durán, Eric author Serna, Naroa author Cano-Garrido, Olivia author Flores, Sandra author Ferrer-Miralles, Neus author Nolan, Verónica author de Marco, Ario author Roher Armentia, Nerea author Unzueta Elorza, Ugutz author Vázquez Gómez, Esther author Villaverde Corrales, Antonio author 2021 Altres ajuts: acords transformatius de la UAB We have developed a simple, robust, and fully transversal approach for thea-la-cartefabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Znions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats. Nanoparticles Protein engineering Divalent cations Protein materials Biomaterials design Biofabrication of functional protein nanoparticles through simple His-tag engineering