2026-04-14T03:12:22Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4578952026-03-13T07:50:41Zcom_2072_98col_2072_378192

urn:hdl:2072/457895 Biofabrication of functional protein nanoparticles through simple His-tag engineering López-Laguna, Hèctor Sanchez, Julieta M. Carratalá, José Vicente Rojas Peña, Mauricio Sánchez-García, Laura Parladé Molist, Eloi Sánchez Chardi, Alejandro Voltà-Durán, Eric Serna, Naroa Cano-Garrido, Olivia Flores, Sandra Ferrer-Miralles, Neus Nolan, Verónica de Marco, Ario Roher Armentia, Nerea Unzueta Elorza, Ugutz Vázquez Gómez, Esther Villaverde Corrales, Antonio Nanoparticles Protein engineering Divalent cations Protein materials Biomaterials design Altres ajuts: acords transformatius de la UAB We have developed a simple, robust, and fully transversal approach for thea-la-cartefabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Znions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats. 2021 Article Agencia Estatal de Investigación BIO2016-76063-R Agencia Estatal de Investigación PID2019-105416RB-I00 Ministerio de Economía y Competitividad RTA2015-00064-C02-02 Agencia Estatal de Investigación PID2019-107298RB-C22 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Instituto de Salud Carlos III PI20/00400 Instituto de Salud Carlos III CP19/00028 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018FI_B2_00051 Agència de Gestió d'Ajuts Universitaris i de Recerca 2019FI_B_00352 Ministerio de Ciencia e Innovación FPU18/04615 ACS sustainable chemistry & engineering ; Vol. 9, Issue 36 (September 2021), p. 12341-12354 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/