2026-04-13T00:03:46Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4563232026-03-26T04:03:11Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Puig Grifol, Núria author Montolio, Lara author Camps-Renom, Pol author Navarra, Laia author Jiménez Altayó, Francesc author Jiménez Xarrié, Elena author Sánchez Quesada, José Luis author Benitez, Sonia author Universitat Autònoma de Barcelona author 2020 Altres ajuts: This research was funded by grants 158/U/2017 from Fundacio La Marato TV3. Electronegative low-density lipoprotein (LDL) (LDL(-)), a modified LDL that is present in blood and exerts atherogenic effects on endothelial cells and monocytes. This study aimed to determine the action of LDL(-) on monocytes differentiated into macrophages. LDL(-) and in vitro-modified LDLs (oxidized, aggregated, and acetylated) were added to macrophages derived from THP1 monocytes over-expressing CD14 (THP1-CD14). Then, cytokine release, cell differentiation, lipid accumulation, and gene expression were measured by ELISA, flow cytometry, thin-layer chromatography, and real-time PCR, respectively. LDL(-) induced more cytokine release in THP1-CD14 macrophages than other modified LDLs. LDL(-) also promoted morphological changes ascribed to differentiated macrophages. The addition of high-density lipoprotein (HDL) and anti-TLR4 counteracted these effects. LDL(-) was highly internalized by macrophages, and it was the major inductor of intracellular lipid accumulation in triglyceride-enriched lipid droplets. In contrast to inflammation, the addition of anti-TLR4 had no effect on lipid accumulation, thus suggesting an uptake pathway alternative to TLR4. In this regard, LDL(-) upregulated the expression of the scavenger receptors CD36 and LOX-1, as well as several genes involved in triglyceride (TG) accumulation. The importance and novelty of the current study is that LDL(-), a physiologically modified LDL, exerted atherogenic effects in macrophages by promoting differentiation, inflammation, and triglyceride-enriched lipid droplets formation in THP1-CD14 macrophages, probably through different receptors. HDL TLR4 Electronegative LDL Foam cells Inflammation Lipid droplets Macrophages Scavenger receptors Triglycerides Electronegative LDL Promotes Inflammation and Triglyceride Accumulation in Macrophages