2026-04-18T04:02:35Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4556742024-11-01T00:07:16Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Chimenti, Laura author Morales-Quinteros, Luis author Puig, Ferranda author Camprubí-Rimblas, Marta author Guillamat-Prats, Raquel author Gómez, Maria Nieves author Tijero, Jessica author Blanch, Lluís author Matute-Bello, Gustavo author Artigas Raventós, Antoni author Universitat Autònoma de Barcelona author 2020 The animal experimental counterpart of human acute respiratory distress syndrome (ARDS) is acute lung injury (ALI). Most models of ALI involve reproducing the clinical risk factors associated with human ARDS, such as sepsis or acid aspiration; however, none of these models fully replicates human ARDS. To compare different experimental animal models of ALI, based on direct or indirect mechanisms of lung injury, to characterize a model which more closely could reproduce the acute phase of human ARDS. Adult male Sprague-Dawley rats were subjected to intratracheal instillations of (1) HCl to mimic aspiration of gastric contents; (2) lipopolysaccharide (LPS) to mimic bacterial infection; (3) HCl followed by LPS to mimic aspiration of gastric contents with bacterial superinfection; or (4) cecal ligation and puncture (CLP) to induce peritonitis and mimic sepsis. Rats were sacrificed 24 h after instillations or 24 h after CLP. At 24 h, rats instilled with LPS or HCl-LPS had increased lung permeability, alveolar neutrophilic recruitment and inflammatory markers (GRO/KC, TNF-α, MCP-1, IL-1β, IL-6). Rats receiving only HCl or subjected to CLP had no evidence of lung injury. Rat models of ALI induced directly by LPS or HCl-LPS more closely reproduced the acute phase of human ARDS than the CLP model of indirectly induced ALI. http://hdl.handle.net/2072/455674 Acute lung injury Animal models Hydrochloric acid Cecal ligation puncture Lipopolysaccharide Comparison of direct and indirect models of early induced acute lung injury