2026-04-17T10:41:44Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4552592024-10-31T05:15:07Zcom_2072_98col_2072_378192

Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria Lin, Yuemei Reino, Clara Carrera, Julian Pérez Cañestro, Julio van Loosdrecht, Mark C. M. Aerobic granular sludge Ammonium-oxidizing bacteria Amyloid Extracellular polymeric substances Glycoproteins A new type of structural extracellular polymers () was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and spectroscopy to characterize specific amino acids and protein secondary structure, and by - with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research. 2018 Article https://ddd.uab.cat/record/227906 urn:10.1002/mbo3.616 urn:oai:ddd.uab.cat:227906 urn:pmid:29604180 urn:pmcid:PMC6291783 urn:pmc-uid:6291783 urn:oai:egreta.uab.cat:publications/6e4918fb-2e02-4f11-a933-9a5edc268898 urn:scopus_id:85044654301 urn:oai:pubmedcentral.nih.gov:6291783 http://hdl.handle.net/2072/455259 eng European Commission 326705 MicrobiologyOpen ; Vol. 7, issue 6 (Dec. 2018) open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/ application/pdf