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00925njm 22002777a 4500 dc Martins, Pedro M. author Navarro, Susanna author Silva, Alexandra author Pinto, Maria F. author Sárkány, Zsuzsa author Figueiredo, Francisco author Barbosa Pereira, Pedro José author Garcia de Carvalho Pinheiro, Francisca author Bednarikova, Zuzana author Burdukiewicz, Michał author Galzitskaya, Oxxana V. author Gazova, Zuzana author Gomes, Cláudio M. author Pastore, Annalisa author Serpell, Louise C. author Skrabana, Rostislav author Smirnovas, Vytautas author Ziaunys, Mantas author Otzen, Daniel E. author Ventura, Salvador author Macedo-Ribeiro, Sandra author Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular author 2020 Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases. Amyloid Reproducible data Protein Peptide Phase separation MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments