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oai:recercat.cat:2072/4368122024-10-31T03:15:53Zcom_2072_98col_2072_378192

Interferometric nanoimmunosensor for label-free and real-time monitoring of Irgarol 1051 in seawater Chocarro Ruiz, Blanca Herranz, Sonia Fernandez-Gavela, Adrian Sanchís, Josep Farre, Marinella Marco, María Pilar Lechuga, Laura M Bimodal waveguide biosensor Competitive immunoassay Environmental monitoring Irgarol 1051 An interferometric nanobiosensor for the specific and label-free detection of the pollutant Irgarol 1051 directly in seawater has been settled. Due to the low molecular weight of Irgarol pollutant and its expected low concentration in seawater, the sensor is based on a competitive inhibition immunoassay. Parameters as surface biofunctionalization, concentration of the selective antibody and regeneration conditions have been carefully evaluated. The optimized immunosensor shows a limit of detection of only 3 ng/L, well below the 16 ng/L set by the EU as the maximum allowable concentration in seawater. It can properly operate during 30 assay-regeneration cycles using the same sensor biosurface and with a time-to-result of only 20 min for each cycle. Moreover, the interferometric nanosensor is able to directly detect low concentrations of Irgarol 1051 in seawater without requiring sample pre-treatments and without showing any background signal due to sea matrix effect. 2018 Article European Commission 614010 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1484 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-418 Ministerio de Economía y Competitividad SEV-2013-0295 Biosensors & bioelectronics ; Vol. 117 (October 2018), p. 47-52 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. https://creativecommons.org/licenses/by-nc-nd/4.0/