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oai:recercat.cat:2072/4362042026-03-13T05:26:58Zcom_2072_98col_2072_378192

The site frequency/dosage spectrum of autopolyploid populations Ferretti, Luca Ribeca, Paolo Ramos Onsins, Sebastián Ernesto Autopolyploidy Dosage distribution Hardy-Weinberg equilibrium High-throughput sequencing Site frequency Neutrality tests Allelic dosage The Site Frequency Spectrum (SFS) and the heterozygosity of allelic variants are among the most important summary statistics for population genetic analysis of diploid organisms. We discuss the generalization of these statistics to populations of autopolyploid organisms in terms of the joint Site Frequency/Dosage Spectrum and its expected value for autopolyploid populations that follow the standard neutral model. Based on these results, we present estimators of nucleotide variability from High-Throughput Sequencing (HTS) data of autopolyploids and discuss potential issues related to sequencing errors and variant calling. We use these estimators to generalize Tajima's D and other SFS-based neutrality tests to HTS data from autopolyploid organisms. Finally, we discuss how these approaches fail when the number of individuals is small. In fact, in autopolyploids there are many possible deviations from the Hardy-Weinberg equilibrium, each reflected in a different shape of the individual dosage distribution. The SFS from small samples is often dominated by the shape of these deviations of the dosage distribution from its Hardy-Weinberg expectations. 2018 Article Agencia Estatal de Investigación AGL2016-78709-R Ministerio de Economía y Competitividad SEV-2015-0533 Frontiers in genetics ; Vol. 9 (Oct. 2018), art. 480 open access Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. https://creativecommons.org/licenses/by/4.0/