2026-04-14T06:07:01Zhttps://recercat.cat/oai/request

oai:recercat.cat:2072/4269692024-10-31T01:45:57Zcom_2072_98col_2072_378192

00925njm 22002777a 4500 dc Ternette, Nicola author Yang, Hongbing author Partridge, Thomas author Llano Montero, Anuska author Cedeño, Samandhy author Fischer, Roman author Charles, Philip D. author Dudek, Nadine L. author Mothe, Beatriz author Crespo Casal, Manuel author Fischer, William M. author Korber, Bette T. M. author Nielsen, Morten author Borrow, Persephone author Purcell, Anthony W. author Brander, Christian author Dorrell, Lucy author Kessler, Benedikt M. author Hanke, Tomáš author Universitat Autònoma de Barcelona author 2015 Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4 + T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development. Cytotoxic T cells Human immunodeficiency virus type I Human leukocyte antigen Immunopeptidome Mass spectrometry Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells