2026-04-13T13:17:43Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.14342/9862025-05-28T12:00:48Zcom_2072_482405com_2072_183628col_2072_482414
Detection of peptide-based nanoparticles in blood plasma by ELISA
Borrós, Salvador
Bode, Gerard H.
Pickl, Karin E.
Sánchez Purrà, Maria
Albaiges, Berta
Pötgens, Andy J. G.
Schmitz, Christoph
Sinner, Frank M.
Losen, Mario
Steinbusch, Harry W. M.
Frank, Hans-Georg
Martinez-Martinez, Pilar
Nanopartícules
Plasma sanguini
Enzimoimmunoassaig
Injeccions intravenoses
Cromatografia de líquids
Espectrometria de masses
Nanoparticles
Blood plasma
Biotin
Enzyme-linked immunoassays
Wistar rats
Acrylics
Intravenous injections
Liquid chromatography-mass spectrometry
Aims
The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.
Materials & Methods
A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology.
Results
The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL.We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS.
Conclusions
We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
2015-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://hdl.handle.net/20.500.14342/986
https://doi.org/10.1371/journal.pone.0126136
eng
PLoS ONE. Vol.10, n.5 (2015), e0126136
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Attribution 4.0 International
© L'autor/a
Public Library of Science (PLoS)