2026-04-17T20:01:41Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.14342/46442025-05-27T14:36:13Zcom_2072_482405com_2072_183628col_2072_482414

00925njm 22002777a 4500 dc Guerle Cavero, Rocío author Balfagon, Alberto author 2023-07-01 The use of animal testing in the cosmetic industry is already prohibited in more than 40 countries, including those of the EU. The pressure for it to be banned worldwide in the future is increasing, so the need for animal alternatives is of great interest today. In addition, using animals and humans in scientific research is ethically reprehensible. This study aimed to prove some of the anti-aging properties of elastin (EL), hydrolyzed collagen (HC), and two vegan collagen-like products (Veg Col) in a tri-layered chitosan membrane that was ionically crosslinked with sodium tripolyphosphate (TPP). In the first approach, as a way of representing different layers of a biological system, such as the epidermis and the two dermis sublayers, EL, HC, or Veg Col were independently introduced into the two inner layers (2L(i+b)). Their effects were compared with those of their introduction into three layers (3L). Different experiments were performed on the membrane to test its elasticity, hydration, moisture retention, and pore reduction at different concentrations of EL, HC, and Veg Col, and the results were normalized vs. a blank membrane. This new alternative to animal or human testing can be suitable for proving certain efficacy claims for active ingredients or products in the pharmaceutical, nutritional, and cosmetic fields. 1422-0067 http://hdl.handle.net/20.500.14342/4644 https://doi.org/10.3390/ijms241311016 Chitosan Collagen Elastin Membrane Elasticity Moisture retention Pore Quitosan Col·lagen Membranes (Biologia) Elasticitat Study of Elastin, Hydrolyzed Collagen and Collagen-like Products in a Tri-Layered Chitosan Membrane to Test Anti-Aging Skin Properties