2026-04-13T01:55:28Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.14342/44752025-05-28T12:00:50Zcom_2072_482405com_2072_183628col_2072_482414

00925njm 22002777a 4500 dc Balmori, Alba author Sandu, Romica author Gheorghe, Daniela author Precupas, Aurica author Tanasescu, Speranta author Sánchez-García, David author Borrós, Salvador author 2021-10-11 The exposure of nanoparticles (NPs) to biological fluids leads to the formation of a protein coating that is known as protein corona (PC). Since PC formation is influenced by the physicochemical properties of the nanoparticles, the understanding of the interplay of the factors that participate in this process is crucial for the development of nanomaterials as cell-targeted delivery vehicles. In general, it is accepted that the PC formation is a complex and dynamic process, which depends on the composition of the medium and the properties of the NP mainly size, shape, and superficial charge. Interestingly, although the interaction between the protein and the NP is essentially a superficial phenomenon, the influence of the roughness of the nanoparticle surface has been scarcely studied. In this work, the influence of superficial roughness and porosity has been studied with the aid of nanodifferential scanning calorimetry (nano-DSC) and isothermal titration calorimetry (ITC) using mesoporous silica nanoparticles (MSNs) as an NP model. The interaction process of the proteins with the NP surface was analyzed by ITC measurements, while the stability and denaturation of the proteins was monitored by nano-DSC. Thanks to the complementarity of these two techniques, a more complete insight into the PC formation on the pores has been accomplished. 2296-4185 http://hdl.handle.net/20.500.14342/4475 https://doi.org/10.3389/fbioe.2021.650281 Protein corona Nano DSC ITC characterization Mesoporous silica nanoparticles (MSN) Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles