2026-04-17T10:14:56Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12328/45402025-05-16T12:47:26Zcom_2072_67741col_2072_484352

00925njm 22002777a 4500 dc Díez Tercero, Leire author Bosch Rué, Èlia author Bosch, Begoña M. author Rojas-Márquez, Raquel author Caballé Serrano, Jordi author Delgado, Luis M. author Pérez, Román A. author 2024 Guided bone regeneration (GBR) is a widely used procedure that prevents the fast in-growth of soft tissues into bone defect. Among the different types of membranes, the use of collagen membranes is the gold standard. However, these membranes are implanted in tissue location where a severe acute inflammation will occur and can be negatively affected. The aim of this study was to develop a collagen-based membrane for GBR that incorporated alginate-hydroxyapatite microparticles. Membranes were manufactured using collagen type I and gelatin and alginate-hydroxyapatite microparticles. Membranes were assessed in terms of topography by scanning electron microscopy and confocal microscopy; stability by swelling after an overnight incubation in saline and enzymatic degradation against collagenase and mechanical properties by tensile tests. Furthermore, the biological response was assessed with SaOs-2 cells and THP-1 macrophages to determine alkaline phosphatase activity and inflammatory cytokine release. Our results showed that the incorporation of different percentages of these microparticles could induce changes in the surface topography. When the biological response was analyzed, either membranes were not cytotoxic to THP-1 macrophages or to SaOs-2 cells and they did not induce the release of pro-inflammatory cytokines. However, the different surface topographies did not induce changes in the macrophage morphology and the release of pro- and anti-inflammatory cytokines, suggesting that the effect of surface roughness on macrophage behavior could be dependent on other factors such as substrate stiffness and composition. Collagen-gelatin membranes with embedded alginate-hydroxyapatite microparticles increased ALP activity, suggesting a positive effect of them on bone regeneration, remaining unaffected the release of pro- and anti-inflammatory cytokines. Díez Tercero, Leire; Bosch Rué, Èlia; Bosch, Begoña M. [et al.]. Engineering a microparticle-loaded rough membrane for guided bone regeneration modulating osteoblast response without inducing inflammation. Colloids and Surfaces B: Biointerfaces, 2024, 241, 113994. Disponible en: <https://www.sciencedirect.com/science/article/abs/pii/S0927776524002534>. Fecha de acceso: 16 ene. 2025. DOI: 10.1016/j.colsurfb.2024.113994 0927-7765 http://hdl.handle.net/20.500.12328/4540 https://dx.doi.org/10.1016/j.colsurfb.2024.113994 Membrana rugosa Regeneració òssia Osteoblasts Inflamació Membrana rugosa Regeneración ósea Osteoblastos Inflamación Rough membrane Bone regeneration Osteoblasts Inflammation Engineering a microparticle-loaded rough membrane for guided bone regeneration modulating osteoblast response without inducing inflammation