2026-04-13T07:30:14Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12328/40302025-05-16T12:51:02Zcom_2072_67741col_2072_484352

00925njm 22002777a 4500 dc Balada, Rafael author Tebé, Cristian author León, Marisol author Arca, Gemma author Alsina, Miguel author Castells, Alba-Aina author Alcántara, Soledad author Garcia-Alix, Alfredo author 2020 Background: We aimed to assess whether a gene expression assay provided insights for understanding the heterogeneity among newborns affected by neonatal encephalopathy (NE). Methods: Analysis by RT-qPCR of the mRNA expression of candidate genes in whole blood from controls (n = 34) and NE (n = 24) patients at <6, 12, 24, 48, 72 and 96 h of life, followed by determination of differences in gene expression between conditions and correlation with clinical variables. Results: During the first 4 days of life, MMP9, PPARG, IL8, HSPA1A and TLR8 were more expressed and CCR5 less expressed in NE patients compared to controls. MMP9 and PPARG increased and CCR5 decreased in moderate/severe NE patients compared to mild. At 6–12 h of life, increased IL8 correlated with severe NE and death, decreased CCR5 correlated with chorioamnionitis and increased HSPA1A correlated with expanded multiorgan dysfunction, severe NE and female sex. Conclusions: MMP9, PPARG and CCR5 mRNA expression within first days of life correlates with the severity of NE. At 6–12 h, IL8 and HSPA1A are good reporters of clinical variables in NE patients. HSPA1A may have a role in the sexual dimorphism observed in NE. CCR5 is potentially involved in the link between severe NE and chorioamnionitis. Balada, Rafael; Tebé, Cristian; León, Marisol [et al.]. Enquiring beneath the surface: can a gene expression assay shed light into the heterogeneity among newborns with neonatal encephalopathy? Pediatric Research, 2020, 88, p. 451-458. Disponible en: <https://www.nature.com/articles/s41390-020-0764-2>. Fecha de acceso: 7 feb. 2024. DOI: 10.1038/s41390-020-0764-2 0031-3998 http://hdl.handle.net/20.500.12328/4030 https://dx.doi.org/10.1038/s41390-020-0764-2 Pediatría Pediatrics Enquiring beneath the surface: can a gene expression assay shed light into the heterogeneity among newborns with neonatal encephalopathy?