2026-04-13T07:21:51Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12328/36682025-05-16T12:50:06Zcom_2072_67741col_2072_484352
RECERCAT
author
Tatullo, Marco
author
Spagnuolo, Gianrico
author
Codispoti, Bruna
author
Zamparini, Fausto
author
Zhang, Anqi
author
Degli Esposti, Micaela
author
Aparicio, Conrado
author
Rengo, Carlo
author
Nuzzolese, Manuel
author
Manzoli, Lucia
author
Fava, Fabio
author
Prati, Carlo
author
Fabbri, Paola
author
Giovanna Gandolfi, Maria
2025-05-16T12:50:06Z
2025-05-16T12:50:06Z
2019
Tatullo, Marco; Spagnuolo, Gianrico; Codispoti, Bruna [et al.]. PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry. Materials, 2019, 12(4), 597. Disponible en: <https://www.mdpi.com/1996-1944/12/4/597>. Fecha de acceso: 19 abr. 2023. DOI: 10.3390/ma12040597
1996-1944
http://hdl.handle.net/20.500.12328/3668
https://dx.doi.org/10.3390/ma12040597
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone.
eng
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Bastides a base d'àcid polilàctic (PLA)
Bastides que contenen silicat de calci
Bastides bioactives
Bastides poroses
Cèl·lules mare mesenquimals de quist periapical humà (hPCy-MSCs)
Odontologia regenerativa
Tècnica de separació de fases induïda tèrmicament (TIPS)
Armazones a base de ácido poliláctico (PLA)
Armazones que contienen silicato de calcio
Andamios bioactivos
Andamios porosos
Células madre mesenquimales de quistes periapicales humanos (hPCy-MSC)
Odontología regenerativa
Técnica de separación de fases inducida térmicamente (TIPS)
Polylactic acid (PLA)-based scaffolds
Calcium silicate-containing scaffolds
Bioactive scaffolds
Porous scaffolds
Human periapical cyst mesenchymal stem cells (hPCy-MSCs)
Regenerative dentistry
Thermally induced phase separation technique (TIPS)
PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry
info:eu-repo/semantics/article