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00925njm 22002777a 4500 dc Godoy-Gallardo, Maria author Mas-Moruno, Carlos author Yu, Kai author Manero, José María author Gil, FJ author Kizhakkedathu, Jayachandran author Rodríguez Rius, Daniel author 2015 Dental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1–11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1–11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment. Godoy Gallardo, Maria; Mas Moruno, Carlos; Yu, Kai [et al.]. Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization. Biomacromolecules, 2015, 16(2), p. 483-496. Disponible en: <https://pubs.acs.org/doi/10.1021/bm501528x#>. Fecha de acceso: 15 nov. 2021. DOI: 10.1021/bm501528x 1525-7797 http://hdl.handle.net/20.500.12328/2946 http://dx.doi.org/10.1021/bm501528x Materials de recobriment Biofilms Pèptids i proteïnes Titani Polimerització radical Implants dentals Materiales de revestimiento Biofilms Péptidos y proteínas Titanio Polimerización radical Implantes dentales Coating materials Biofilms Peptides and proteins Titanium Radical polymerization Dental implants Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization