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oai:recercat.cat:20.500.12327/9862025-10-22T11:27:24Zcom_2072_4428com_2072_4427col_2072_487898

2025-10-22T11:27:24Z urn:hdl:20.500.12327/986 Cosmopolitan distribution of Endozoicomonas-like organisms and other intracellular microcolonies of bacteria causing infection in marine molluscs Cano, Irene Ryder, David Webb, Steve C. Jones, Brian J. Brosnahan, Cara L. Carrasco, Noelia Bordinier, Barbara Furones, Dolors Pretto, Tobia Carella, Francesca Chollet, Bruno Arzul, Isabelle Cheslett, Deborah Collins, Evelyn Lohrmann, Karin B. Valdivia, Ana L. Ward, Georgia Carballal Durán, María Jesús Villalba García, Antonio Marigomez, Ionan Mortensen, Stein Christison, Kevin Kevin, Wakeman C. Bustos, Eduardo Christie, Lyndsay Green, Matthew Feist, Stephen W. Producció Animal Aqüicultura Intracellular microcolonies of bacteria, in some cases developing large extracellular cysts, have been historically reported infecting a wide diversity of economically important mollusc species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterise those organisms, traditionally named as Rickettsia or Chlamydia -like organisms (RLO/CLO), via international collaboration, 98 samples comprising 20 mollusc species were collected over 10 countries and examined using histology and phylogenetic analysis. A 16S rRNA gene amplicon library-based sequencing showed the presence of different species of Endozoicomonas-like organisms (ELO) in all the mollusc species analysed, infecting primarily gill and digestive glands. Co-infections of ELOs with other intracellular bacteria were also observed. Subsequent phylogenetic analysis of Operational Taxonomic Units (OTU) revealed a novel microbial diversity associated with molluscan RLO/CLOs infection distributed along different taxa, including Spirochaetes phyla, Rickettsiales order, Simkaniaceae family, Mycoplasma and Francisella genera, and sulfur-oxidizing endosymbionts. Sequences like Francisella halioticida/philomiragia and Candidatus Brownia rhizoecola were also obtained. The presence of ELO sequences in the RLO/CLO infection was confirmed by standard PCR, Sanger sequencing, and by in situ hybridisation in a selection of samples. The phylogenetic analysis conducted in this study will allow for further characterization of the microbial community associated with Rickettsia and Chlamydia-like infection in marine molluscs and their correlation with severity of the lesions in order to reveal their role as endosymbionts, commensals or true pathogens. 2025-10-22T11:27:24Z 2025-10-22T11:27:24Z 2020-10-30 info:eu-repo/semantics/article Cano, Irene, David Ryder, Steve C. Webb, Brian J. Jones, Cara L. Brosnahan, Noelia Carrasco, and Barbara Bodinier et al. 2020. "Cosmopolitan Distribution Of Endozoicomonas-Like Organisms And Other Intracellular Microcolonies Of Bacteria Causing Infection In Marine Mollusks". Frontiers In Microbiology 11. doi:10.3389/fmicb.2020.577481. 1664-302X http://hdl.handle.net/20.500.12327/986 https://doi.org/10.3389/fmicb.2020.577481 eng Frontiers in Microbiology EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International Frontiers Media