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00925njm 22002777a 4500 dc Toldrà, Anna author O'Sullivan, Ciara K. author Diogène, Jorge author Campàs, Mònica author 2020-08-10 Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nucleic acid-based (molecular) methods are being considered for the unequivocal identification of microalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nucleic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process (from sample procurement to in-situ implementation) are described. Toldrà, Anna, Ciara K. O'Sullivan, Jorge Diogène, and Mònica Campàs. 2020. "Detecting Harmful Algal Blooms With Nucleic Acid Amplification-Based Biotechnological Tools". Science Of The Total Environment 749: 141605. doi:10.1016/j.scitotenv.2020.141605. 0048-9697 http://hdl.handle.net/20.500.12327/957 https://doi.org/10.1016/j.scitotenv.2020.141605 Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools