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oai:recercat.cat:20.500.12327/9572025-10-22T11:02:15Zcom_2072_4428com_2072_4427col_2072_487898

2025-10-22T11:02:15Z urn:hdl:20.500.12327/957 Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools Toldrà, Anna O'Sullivan, Ciara K. Diogène, Jorge Campàs, Mònica Producció Animal Aigües Marines i Continentals Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nucleic acid-based (molecular) methods are being considered for the unequivocal identification of microalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nucleic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process (from sample procurement to in-situ implementation) are described. 2025-10-22T11:02:15Z 2025-10-22T11:02:15Z 2020-08-10 info:eu-repo/semantics/article Toldrà, Anna, Ciara K. O'Sullivan, Jorge Diogène, and Mònica Campàs. 2020. "Detecting Harmful Algal Blooms With Nucleic Acid Amplification-Based Biotechnological Tools". Science Of The Total Environment 749: 141605. doi:10.1016/j.scitotenv.2020.141605. 0048-9697 http://hdl.handle.net/20.500.12327/957 https://doi.org/10.1016/j.scitotenv.2020.141605 eng Science of The Total Environment MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanalíticos para detección rápida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSISNG http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier